Exhibit 21.01

SUBSIDIARIES OF KEYNOTE SYSTEMS, INC.

Velogic, Inc., a California corporation

Keynote Europe Limited, a corporation organized under the laws of the England

Keynote Canada, a corporation organized under the laws of Canada

Vividence Corporation, a California corporation

Keynote SIGOS GmbH, a corporation organized under the laws of Germany

Keynote German Management GmbH

Keynote German Holding Company GmbH