EXHIBIT 23

Consent of Independent Registered Public Accounting Firm

We consent to the incorporation by reference in the Registration Statements (Forms S-8 No. 33-29136 and No. 333-160652) pertaining to the CSX Corporation Capital Builder Plan of our report dated June 20, 2024, with respect to the financial statements and supplemental schedules of the CSX Corporation Capital Builder Plan included in this Annual Report (Form 11-K) for the year ended December 31, 2023.

/s/ Ernst & Young LLP

Jacksonville, Florida

June 20, 2024

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