Exhibit 10.1

AquaBountyTechnologies,Inc.

Amendment No. 3 to the AquaBountyTechnologies,Inc.

2016EquityIncentivePlan

AquaBounty Technologies,Inc.,a Delaware corporation (the"Company"), by action of itsBoardofDirectorstakeninaccordance withtheauthority granted toitbySection2(b)(vii)of theAquaBounty TechnologiesInc.2016 Equity Incentive Plan(the"Plan"), hereby amends the Plan in thefollowing respect effective May 25,2023:

DeleteSection3(a)ofthe2016 EIPandreplaceitwiththefollowingtext:

a. Share Reserve. Subject to Section9(a) relating to Capitalization Adjustment, the aggregate number of shares of Common Stock that may be issued pursuant toAwards will not exceed 4,300,000 shares (the “Share Reserve”).

Delete Section 2(b)(xi) of the 2016 EIP and replace it with the following text:

b. subject the approval of the Company’s stockholders and with the consent of any adversely affected Participant, (A) the reduction of the exercise, purchase, or strike price of any outstanding Award; (B) the cancellation of any outstanding Award and the grant in substitution therefor of a new Option, SAR, Restricted Stock Award, Restricted Stock Unit Award, Other Award, cash, or other valuable consideration determined by the Board, in its sole discretion, with any such substituted award (I) covering the same or a different number of shares of Common Stock as the cancelled Award and (II) granted under the Plan or another equity or compensatory plan of the Company; or (C) any other action that is treated as a repricing under generally accepted accounting principles.

INWITNESSWHEREOF,AquaBounty Technologies,Inc.hascausedthisinstrumentto besigned inits name by a duly authorized officer on this May 25,2023.

AquaBountyTechnologies,Inc.

By:   /s/Angela M. Olsen

Angela M.Olsen

GeneralCounselandSecretary