Exhibit 16.1

Deloitte Touche Tohmatsu ABN 74 490 121 060 11 Waymouth Street Adelaide, SA, 5000 Australia   Tel: +61 8 8407 7000 www.deloitte.com.au

19 November 2021

Securities and Exchange Commission

100 F Street, N.E.

Washington, D.C. 20549-7561

Dear Sirs/Madams,

We have read the Item titled “Change in Accountants” of Bionomics Limited’s Prospectus and Registration Statement, and we agree with the statements made therein.

Yours sincerely

DELOITTE TOUCHE TOHMATSU

/s/ Deloitte Touche Tohmatsu

Lee Girolamo

Partner

Chartered Accountants

Adelaide, South Australia

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Liability limited by a scheme approved under Professional Standards Legislation. Member of Deloitte Asia Pacific Limited and the Deloitte organisation.

CONFIDENTIAL